Products

Product Features

• 1. Rapid Decalcification: Most bone samples can be decalcified within 12 to 24 hours.
• 2. Sample Protection: Preserves DNA, RNA, and protein components within the cells of bone samples.
• 3. Good Staining Results: Minimizes impact on tissue, ensuring optimal staining outcomes.

Product Introduction

• Product name：PVB Gill1 Hematoxylin
  Cat：PVB-3006-001(500mL)
  　　  PVB-3006-002(1000mL)
  preservation condition：Store at room temperature away from light for 12 months

Product Introduction

Product name：RNAkeeper 
Cat：PVB-3004 (100mL)
         PVB-3005 (500ml)
Storage Conditions: Store at room temperature. Valid for 12 months. If precipitation occurs, place the solution at 37℃ until the precipitate dissolves, it can be used normally then.

Product Description

RNAkeeper is a liquid, non-toxic tissue preservation solution. It can rapidly penetrate into tissue cells and protect and stabilize the RNA within them. It ensures that the quantity and quality of RNA in tissue cells not preserved at ultra-low temperatures are fully protected. RNAkeeper can reduce the need to immediately place tissue samples in liquid nitrogen for RNA preservation after acquisition (see Figures 1 and 2). RNAkeeper can be widely applied to various animal tissue samples, such as brain, heart, liver, lung, kidney, spleen, fat, muscle, and glands. RNAkeeper is also effective for fruit fly and some plant tissues. RNAkeeper is compatible with most RNA extraction reagents, such as TRI Reagent and RNAqueous, etc.

Figure 1 shows the results of RNA electrophoresis after extraction from mouse spleen tissues that were:
Stored in RNAkeeper at 4℃ for 4 weeks (Figure 1, left),
Immediately placed in liquid nitrogen after dissection (Figure 1, middle),
Stored in PBS at 4℃ for 4 weeks (Figure 1, right).

Figure 2 shows the results of RNA electrophoresis after extraction from mouse spleen tissues that were:
Stored in RNAkeeper at room temperature for 2 weeks (Figure 2, left),
Immediately placed in liquid nitrogen after dissection (Figure 2, middle),
Stored in PBS at room temperature for 2 weeks (Figure 2, right).

Method of application

Tissue Blocks:
RNAkeepe is only applicable to fresh tissues. There is no need to freeze the tissue before immersing it in RNAkeeper. Cut the tissue block to a thickness of less than or equal to 0.5 cm, and then place the tissue into five times its volume of RNAkeeper for storage. RNAkeeper will not dissolve or destroy the structural integrity of the tissue sample. If necessary, tissue that has been immersed in RNAkeeper can be sectioned, and then re-immersed in RNAkeeper. Small organs, such as mouse liver, kidneys, and spleen, can be preserved intact in RNAkeeper (see “Sample Preservation Time in the Preservation Solution” for details).
Plant Tissues:
It is necessary to disrupt their natural barriers that prevent diffusion, such as the waxy cuticle of leaf tissues, to allow RNAkeeper to penetrate into the tissue cells (see “Sample Preservation Time in the Preservation Solution” for details).

The storage time of the sample in the storage solution

Store at -80℃
This method is recommended for the processing of long-term stored samples. The sample is soaked in a sufficient amount (at least five times the volume) of RNAkeeper, incubated at 4 ° C overnight, then removed from the RNAkeeper tissue preservation solution and stored at -80 ° C. Samples can be thawed and refrozen at room temperature in subsequent operations without affecting the quality and quantity of RNA.

Store at -20℃
This method is recommended for the processing of samples intended for long-term preservation. Immerse the sample in an adequate volume of RNAkeeper, and incubate it overnight at 4°C. After incubation, transfer the sample directly to -20°C. At -20°C, RNAkeeper will not solidify, but crystallization may occur. This does not affect subsequent RNA extraction. The sample can be thawed and refrozen at room temperature during subsequent operations without compromising the quality and quantity of RNA.

Store at 4℃ and at room temperature
The sample can be preserved in RNAkeeper at 4°C for one month without RNA degradation.
Experiments have shown that the sample can be preserved in RNAkeeper for two weeks without RNA degradation.

RNA extraction from samples in storage solution

Using sterilized forceps, remove the sample from the RNAkeeper and transfer it into the RNA extraction solution, followed by rapid homogenization.

Cell FFPE Controls

Stable and consistent immunohistochemical (IHC) staining can reveal the spatial information of biomarkers in tissues and cells, which is crucial in clinical pathology diagnosis, drug development, and translational medicine. However, IHC staining results can vary due to factors such as sample preparation, reagent batches, experimental operations, and the performance of automated instrument platforms. These variations can affect the interpretation and evaluation of the results. In routine pathological testing, the lack of renewable IHC standards significantly increases medical costs. Compared with IHC, the analytical error rates in clinical chemistry, immunology, and hematology are one order of magnitude lower (<1%).

Product Introduction

Stable and consistent immunohistochemical (IHC) staining can reveal the spatial information of biomarkers in tissues and cells, which is crucial in clinical pathology diagnosis, drug development, and translational medicine. However, IHC staining results can vary due to factors such as sample preparation, reagent batches, experimental operations, and the performance of automated instrument platforms. These variations can affect the interpretation and evaluation of the results.
In routine pathological testing, the lack of renewable IHC standards significantly increases medical costs. Compared with IHC, the analytical error rates in clinical chemistry, immunology, and hematology are one order of magnitude lower (<1%).

Beijing PhenoVision Biotechnology Co., Ltd. has introduced advanced Cell FFPE Control preparation processes, ensuring a high degree of consistency in cell numbers between paraffin blocks and among each section of a single paraffin block. This enables the company to provide industrial-grade quality control standards for routine pathological testing, such as immunohistochemistry (IHC), in situ hybridization (ISH), and PCR.

Product Advantages

1. High Density, High Homogeneity: The process of creating high-density, high-homogeneity cell paraffin blocks and sections ensures consistent staining results between sections and across batches.

2. Controllable Antigen Expression Levels: Pre-prepared cell controls with specific antigen expression levels are available for comparison with tissue samples under the same staining conditions during immunohistochemical (IHC) assays.

3. Cell Array Quality Control System: The cell array quality control system includes cell arrays with positive or graded positive expressions for different antibody targets, which can be used for semi-quantitative or qualitative analysis of staining results.

Product list

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Show Cases

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A proprietary on-slide-control system for companion diagnostics aids in pathological diagnosis.

Calibration of differences in antibody clones, batch variations of antibodies, dilution concentrations, and experimental procedural variations to address discrepancies in detection results.

2. HER2 4in1 Tissue Control

It can be used to evaluate information such as the performance of instruments, experimental procedures, reagent quality, and the consistency of experimental results during the HER2 immunohistochemical staining process.